Cloning, soluble expression, and purification of the RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae

We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The...

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Vydáno v:Bioengineered
Hlavní autoři: Chhetri, Gaurav, Ghosh, Arabinda, Chinta, Ramesh, Akhtar, Sohail, Tripathi, Timir
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis 2015
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4601343/
https://ncbi.nlm.nih.gov/pubmed/25551420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2014.1002301
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