Gene replacement in Halobacterium halobium and expression of bacteriorhodopsin mutants.
A gene replacement method has been developed to express bacteriorhodopsin mutants in the archaeon Halobacterium halobium. Selectable plasmids carrying the bacterioopsin gene (bop) were integrated at the chromosomal bop locus of H. halobium. Under nonselective conditions, recombinants were isolated t...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46005/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8446619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.5.1987 |
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