Gene replacement in Halobacterium halobium and expression of bacteriorhodopsin mutants.

A gene replacement method has been developed to express bacteriorhodopsin mutants in the archaeon Halobacterium halobium. Selectable plasmids carrying the bacterioopsin gene (bop) were integrated at the chromosomal bop locus of H. halobium. Under nonselective conditions, recombinants were isolated t...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Krebs, M P, Mollaaghababa, R, Khorana, H G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46005/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8446619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.5.1987
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