Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics

Leguminous lectins have a conserved carbohydrate recognition site comprising four loops (A–D). Here, we randomly mutated the sequence and length of loops C and D of peanut agglutinin (PNA) and expressed the proteins on the surface of mouse green fluorescent protein (GFP)-reporter cells. Flow cytomet...

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Veröffentlicht in:Biomolecules
Hauptverfasser: Soga, Keisuke, Abo, Hirohito, Qin, Sheng-Ying, Kyoutou, Takuya, Hiemori, Keiko, Tateno, Hiroaki, Matsumoto, Naoki, Hirabayashi, Jun, Yamamoto, Kazuo
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4598763/
https://ncbi.nlm.nih.gov/pubmed/26287256
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom5031540
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