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Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics
Leguminous lectins have a conserved carbohydrate recognition site comprising four loops (A–D). Here, we randomly mutated the sequence and length of loops C and D of peanut agglutinin (PNA) and expressed the proteins on the surface of mouse green fluorescent protein (GFP)-reporter cells. Flow cytomet...
Enregistré dans:
| Publié dans: | Biomolecules |
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| Auteurs principaux: | , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MDPI
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4598763/ https://ncbi.nlm.nih.gov/pubmed/26287256 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom5031540 |
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