MStern Blotting–High Throughput Polyvinylidene Fluoride (PVDF) Membrane-Based Proteomic Sample Preparation for 96-Well Plates

We describe a 96-well plate compatible membrane-based proteomic sample processing method, which enables the complete processing of 96 samples (or multiples thereof) within a single workday. This method uses a large-pore hydrophobic PVDF membrane that efficiently adsorbs proteins, resulting in fast l...

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Vydáno v:Mol Cell Proteomics
Hlavní autoři: Berger, Sebastian T., Ahmed, Saima, Muntel, Jan, Cuevas Polo, Nerea, Bachur, Richard, Kentsis, Alex, Steen, Judith, Steen, Hanno
Médium: Artigo
Jazyk:Inglês
Vydáno: The American Society for Biochemistry and Molecular Biology 2015
Témata:
Technological Innovations and Resources
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4597154/
https://ncbi.nlm.nih.gov/pubmed/26223766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.049650
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