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Selective Chemoprecipitation to Enrich Nitropeptides from Complex Proteomes for Mass Spectrometric Analysis

Posttranslational protein nitration has attracted interest due to its involvement in cellular signaling, effects on protein function, and as a potential biomarker of nitroxidative stress. We describe a procedure for enriching nitropeptides for mass spectrometry-based proteomics that is a simple and...

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Détails bibliographiques
Publié dans:Nat Protoc
Auteurs principaux: Prokai, Laszlo, Guo, Jia, Prokai-Tatrai, Katalin
Format: Artigo
Langue:Inglês
Publié: 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4594882/
https://ncbi.nlm.nih.gov/pubmed/24651500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.052
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