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Selective Chemoprecipitation to Enrich Nitropeptides from Complex Proteomes for Mass Spectrometric Analysis

Posttranslational protein nitration has attracted interest due to its involvement in cellular signaling, effects on protein function, and as a potential biomarker of nitroxidative stress. We describe a procedure for enriching nitropeptides for mass spectrometry-based proteomics that is a simple and...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Protoc
Hauptverfasser: Prokai, Laszlo, Guo, Jia, Prokai-Tatrai, Katalin
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4594882/
https://ncbi.nlm.nih.gov/pubmed/24651500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.052
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