Nano-mole Scale Sequential Signal Assignment by (1)H-Detected Protein Solid-state NMR using Ultra-Fast Magic-Angle Spinning and HIGHLIGHT Spectral Editing

We present a 3D (1)H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10–100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ~80 kHz with a novel spectral-editing method, which permits drastic spectral simplification. The approach offers...

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Foilsithe in:Chem Commun (Camb)
Main Authors: Wang, Songlin, Parthasarathy, Sudhakar, Xiao, Yiling, Nishiyama, Yusuke, Long, Fei, Matsuda, Isamu, Endo, Yuki, Nemoto, Takahiro, Yamauchi, Kazuo, Asakura, Tetsuo, Takeda, Mitsuhiro, Terauchi, Tsutomu, Kainosho, Masatsune, Ishii, Yoshitaka
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2015
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Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4589527/
https://ncbi.nlm.nih.gov/pubmed/26317132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c5cc04618a
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