Nano-Mole Scale Side-Chain Signal Assignment by (1)H-Detected Protein Solid-State NMR by Ultra-Fast Magic-Angle Spinning and Stereo-Array Isotope Labeling

We present a general approach in (1)H-detected (13)C solid-state NMR (SSNMR) for side-chain signal assignments of 10-50 nmol quantities of proteins using a combination of a high magnetic field, ultra-fast magic-angle spinning (MAS) at ~80 kHz, and stereo-array-isotope-labeled (SAIL) proteins [Kainos...

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Опубликовано в: :PLoS One
Главные авторы: Wang, Songlin, Parthasarathy, Sudhakar, Nishiyama, Yusuke, Endo, Yuki, Nemoto, Takahiro, Yamauchi, Kazuo, Asakura, Tetsuo, Takeda, Mitsuhiro, Terauchi, Tsutomu, Kainosho, Masatsune, Ishii, Yoshitaka
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2015
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Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4391754/
https://ncbi.nlm.nih.gov/pubmed/25856081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0122714
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