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Determining exon connectivity in complex mRNAs by nanopore sequencing
Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 ‘full-l...
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| Veröffentlicht in: | Genome Biol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4588896/ https://ncbi.nlm.nih.gov/pubmed/26420219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0777-z |
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