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Determining exon connectivity in complex mRNAs by nanopore sequencing

Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 ‘full-l...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Bolisetty, Mohan T., Rajadinakaran, Gopinath, Graveley, Brenton R.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4588896/
https://ncbi.nlm.nih.gov/pubmed/26420219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0777-z
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