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Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

Understanding the extent to which enzyme evolution is reversible can shed light on the fundamental relationship between protein sequence, structure, and function. Here, we perform an experimental test of evolutionary reversibility using directed evolution from a phosphotriesterase to an arylesterase...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Kaltenbach, Miriam, Jackson, Colin J, Campbell, Eleanor C, Hollfelder, Florian, Tokuriki, Nobuhiko
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4579389/
https://ncbi.nlm.nih.gov/pubmed/26274563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.06492
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