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Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette

A central goal in the development of genome engineering technology is to reduce the time and labor required to produce custom genome modifications. Here we describe a new selection strategy for producing fluorescent protein (FP) knock-ins using CRISPR/Cas9-triggered homologous recombination. We have...

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Detalhes bibliográficos
Publicado no:Genetics
Main Authors: Dickinson, Daniel J., Pani, Ariel M., Heppert, Jennifer K., Higgins, Christopher D., Goldstein, Bob
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4574250/
https://ncbi.nlm.nih.gov/pubmed/26044593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.178335
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