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Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette
A central goal in the development of genome engineering technology is to reduce the time and labor required to produce custom genome modifications. Here we describe a new selection strategy for producing fluorescent protein (FP) knock-ins using CRISPR/Cas9-triggered homologous recombination. We have...
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Publicado no: | Genetics |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Genetics Society of America
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4574250/ https://ncbi.nlm.nih.gov/pubmed/26044593 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.178335 |
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