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Confocal Microscopy Reveals Zones of Membrane Remodeling in the Outer Cortex of the Human Lens
PURPOSE: To optimize fixation, sectioning, and immunolabeling protocols to map the morphology of the human lens with confocal microscopy. METHODS: Transparent human lenses were fixed in 0.75% paraformaldehyde for 24 hours, cut in half, and fixed for another 24 hours. Lenses were cryoprotected, secti...
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| 出版年: | Invest Ophthalmol Vis Sci |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4572844/ https://ncbi.nlm.nih.gov/pubmed/19357350 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/iovs.09-3435 |
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