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Systematic Evaluation of Bioorthogonal Reactions in Live Cells with Clickable HaloTag Ligands: Implications for Intracellular Imaging
[Image: see text] Bioorthogonal reactions, including the strain-promoted azide–alkyne cycloaddition (SPAAC) and inverse electron demand Diels–Alder (iEDDA) reactions, have become increasingly popular for live-cell imaging applications. However, the stability and reactivity of reagents has never been...
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Publicado no: | J Am Chem Soc |
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Main Authors: | , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Chemical
Society
2015
|
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4572613/ https://ncbi.nlm.nih.gov/pubmed/26270632 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b06847 |
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