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Expression of human estrogen receptor mutants in Xenopus oocytes: correlation between transcriptional activity and ability to form protein-DNA complexes.

The human estrogen receptor (hER) is a trans-acting regulatory protein composed of a series of discrete functional domains. We have microinjected an hER expression vector (HEO) into Xenopus oocyte nuclei and demonstrate, using Western blot assay, that the hER is synthesized. When nuclear extracts fr...

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Detalhes bibliográficos
Publicado no:EMBO J
Principais autores: Theulaz, I, Hipskind, R, ten Heggeler-Bordier, B, Green, S, Kumar, V, Chambon, P, Wahli, W
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC457149/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3169000/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02992.x
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