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Expression of human estrogen receptor mutants in Xenopus oocytes: correlation between transcriptional activity and ability to form protein-DNA complexes.

The human estrogen receptor (hER) is a trans-acting regulatory protein composed of a series of discrete functional domains. We have microinjected an hER expression vector (HEO) into Xenopus oocyte nuclei and demonstrate, using Western blot assay, that the hER is synthesized. When nuclear extracts fr...

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Dades bibliogràfiques
Publicat a:EMBO J
Autors principals: Theulaz, I, Hipskind, R, ten Heggeler-Bordier, B, Green, S, Kumar, V, Chambon, P, Wahli, W
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1988
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC457149/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3169000/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02992.x
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