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Expression of human estrogen receptor mutants in Xenopus oocytes: correlation between transcriptional activity and ability to form protein-DNA complexes.

The human estrogen receptor (hER) is a trans-acting regulatory protein composed of a series of discrete functional domains. We have microinjected an hER expression vector (HEO) into Xenopus oocyte nuclei and demonstrate, using Western blot assay, that the hER is synthesized. When nuclear extracts fr...

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Detalhes bibliográficos
Main Authors: Theulaz, I, Hipskind, R, ten Heggeler-Bordier, B, Green, S, Kumar, V, Chambon, P, Wahli, W
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC457149/
https://ncbi.nlm.nih.gov/pubmed/3169000
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