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Folding optimization in vivo uncovers new chaperones

By employing a genetic selection that forces the cell to fold an unstable, aggregation prone test protein in order to survive, we have generated bacterial strains with enhanced periplasmic folding capacity. These strains enhance the soluble steady state level of the test protein. Most of the bacteri...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Mol Biol
Prif Awduron: Lennon, Christopher W, Thamsen, Maike, Friman, Elias T, Cacciaglia, Austin, Sachsenhauser, Veronika, Sorgenfrei, Frieda A, Wasik, Milena A, Bardwell, James CA
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2015
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4569523/
https://ncbi.nlm.nih.gov/pubmed/26003922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.05.013
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