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Folding optimization in vivo uncovers new chaperones

By employing a genetic selection that forces the cell to fold an unstable, aggregation prone test protein in order to survive, we have generated bacterial strains with enhanced periplasmic folding capacity. These strains enhance the soluble steady state level of the test protein. Most of the bacteri...

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Bibliografiska uppgifter
I publikationen:J Mol Biol
Huvudupphovsmän: Lennon, Christopher W, Thamsen, Maike, Friman, Elias T, Cacciaglia, Austin, Sachsenhauser, Veronika, Sorgenfrei, Frieda A, Wasik, Milena A, Bardwell, James CA
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4569523/
https://ncbi.nlm.nih.gov/pubmed/26003922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.05.013
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