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Optimizing Thiophosphorylation in the Presence of Competing Phosphorylation with MALDI-TOF–MS Detection

Thiophosphorylation provides a metabolically stable, chemically reactive phosphorylation analogue for analyzing the phosphoproteome in vitro and in vivo. We developed a MALDI-TOF–MS based assay for optimizing thiophosphopeptide production by a kinase even in the presence of Mg(2+) and ATP. We found...

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書目詳細資料
發表在:J Proteome Res
Main Authors: Parker, Laurie L., Schilling, Alexander B., Kron, Stephen J., Kent, Stephen B. H.
格式: Artigo
語言:Inglês
出版: 2005
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4568822/
https://ncbi.nlm.nih.gov/pubmed/16212443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr050150e
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