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Kinetic Detection of Orthogonal Protein and Chemical Coordinates in Enzyme Catalysis: Double Mutants of Soybean Lipoxygenase

[Image: see text] Soybean lipoxygenase-1 (SLO-1) is a paradigmatic enzyme system for studying the contribution of hydrogen tunneling to enzymatic proton-coupled electron transfer processes. In this study, the impact of pairs of double mutants on the properties of SLO-1 is presented. Steady-state rat...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Sharma, Sudhir C., Klinman, Judith P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2015
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4565126/
https://ncbi.nlm.nih.gov/pubmed/26154975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00374
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