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A time-resolved FRET assay to measure activity of the deamidase of the prokaryotic ubiquitin-like protein
The modification of proteins in Mycobacterium tuberculosis (Mtb) by the prokaryotic ubiquitin-like protein (Pup) targets them for degradation by mycobacterial proteasomes. Although functionally similar to eukaryotic deubiquitylating enzymes, the deamidase of Pup (Dop) has no known mammalian homologs...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4560620/ https://ncbi.nlm.nih.gov/pubmed/26205584 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.07.003 |
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