A time-resolved FRET assay to measure activity of the deamidase of the prokaryotic ubiquitin-like protein

The modification of proteins in Mycobacterium tuberculosis (Mtb) by the prokaryotic ubiquitin-like protein (Pup) targets them for degradation by mycobacterial proteasomes. Although functionally similar to eukaryotic deubiquitylating enzymes, the deamidase of Pup (Dop) has no known mammalian homologs...

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Bibliographic Details
Published in:Anal Biochem
Main Authors: Eustis, Ian C., Huang, Jessica, Pilkerton, Meagan E., Whedon, Samuel D., Chatterjee, Champak
Format: Artigo
Language:Inglês
Published: 2015
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4560620/
https://ncbi.nlm.nih.gov/pubmed/26205584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.07.003
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