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PEA-CLARITY: 3D molecular imaging of whole plant organs

Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Palmer, William M., Martin, Antony P., Flynn, Jamie R., Reed, Stephanie L., White, Rosemary G., Furbank, Robert T., Grof, Christopher P. L.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4556961/
https://ncbi.nlm.nih.gov/pubmed/26328508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13492
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