Pig oocyte activation utilizing a Zn(2+) chelator, TPEN

Artificial oocyte activation is a critical step during somatic cell nuclear transfer (SCNT). Most of current activation protocols focus on inducing an increase in the intracellular free Ca(2+) concentration of the oocyte. Here, we have utilized a zinc chelator, TPEN, to enhance the efficiency of ooc...

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Publicado en:Theriogenology
Main Authors: Lee, Kiho, Davis, Alyssa, Zhang, Lu, Ryu, Junghyun, Spate, Lee D, Park, Kwang-Wook, Samuel, Melissa S, Walters, Eric M, Murphy, Clifton N, Machaty, Zoltan, Prather, Randall S
Formato: Artigo
Idioma:Inglês
Publicado: 2015
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4553067/
https://ncbi.nlm.nih.gov/pubmed/26143360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2015.05.036
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