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Pig oocyte activation utilizing a Zn(2+) chelator, TPEN
Artificial oocyte activation is a critical step during somatic cell nuclear transfer (SCNT). Most of current activation protocols focus on inducing an increase in the intracellular free Ca(2+) concentration of the oocyte. Here, we have utilized a zinc chelator, TPEN, to enhance the efficiency of ooc...
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| Publicado no: | Theriogenology |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4553067/ https://ncbi.nlm.nih.gov/pubmed/26143360 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2015.05.036 |
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