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An Engineered Split Intein for Photoactivated Protein Trans-Splicing

Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the...

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Publicat a:PLoS One
Autors principals: Wong, Stanley, Mosabbir, Abdullah A., Truong, Kevin
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4552755/
https://ncbi.nlm.nih.gov/pubmed/26317656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0135965
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