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Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was overproduced in a T7 expression vector in Escherichia coli. The corresponding polypeptide of 68 amino acids was purified to homogeneity. The homeodomain was analysed by ultracentrifugation and assayed for DNA binding. The seco...

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Bibliografiske detaljer
Main Authors: Müller, M, Affolter, M, Leupin, W, Otting, G, Wüthrich, K, Gehring, W J
Format: Artigo
Sprog:Inglês
Udgivet: 1988
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC455145/
https://ncbi.nlm.nih.gov/pubmed/2907479
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