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Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was overproduced in a T7 expression vector in Escherichia coli. The corresponding polypeptide of 68 amino acids was purified to homogeneity. The homeodomain was analysed by ultracentrifugation and assayed for DNA binding. The seco...

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Publicat a:EMBO J
Autors principals: Müller, M, Affolter, M, Leupin, W, Otting, G, Wüthrich, K, Gehring, W J
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1988
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC455145/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2907479/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb03328.x
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