Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells

Maintenance and differentiation of human pluripotent stem cells (hPSCs) usually requires culture on a substrate for cell adhesion. A commonly used substratum is Matrigel purified from Engelbreth—Holm—Swarm sarcoma cells, and consists of a complex mixture of extracellular matrix proteins, proteoglyca...

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Udgivet i:PLoS One
Main Authors: Nagaoka, Masato, Kobayashi, Motohiro, Kawai, Chie, Mallanna, Sunil K., Duncan, Stephen A.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4550348/
https://ncbi.nlm.nih.gov/pubmed/26308339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0136350
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