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Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering

The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertio...

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Dettagli Bibliografici
Pubblicato in:Appl Environ Microbiol
Autori principali: Derntl, Christian, Kiesenhofer, Daniel P., Mach, Robert L., Mach-Aigner, Astrid R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542242/
https://ncbi.nlm.nih.gov/pubmed/26150462
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01545-15
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