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Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering
The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertio...
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Publicado no: | Appl Environ Microbiol |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4542242/ https://ncbi.nlm.nih.gov/pubmed/26150462 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01545-15 |
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