Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus

BACKGROUND: Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease, as well as hepatitis-splenomegaly syndrome in chickens. To date, conventional reverse transcriptase polymerase chain reaction (RT-PCR) and nested RT-PCR methods have been used for the diagnosis of...

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Veröffentlicht in:BMC Vet Res
Hauptverfasser: Zhao, Qin, Xie, Sha, Sun, Yani, Chen, Yiyang, Gao, Jiming, Li, Huiya, Wang, Xinjie, Syed, Shahid Faraz, Liu, Baoyuan, Wang, Lizhen, Zhang, Gaiping, Zhou, En-Min
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2015
Schlagworte:
Methodology Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542097/
https://ncbi.nlm.nih.gov/pubmed/26260476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12917-015-0507-5
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