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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Traditional vaccine adjuvants, such as alum, elicit suboptimal CD8+ T cell responses. To address this major challenge in vaccine development, various nanoparticle systems have been engineered to mimic features of pathogens to improve antigen delivery to draining lymph nodes and increase antigen upta...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ochyl, Lukasz J., Moon, James J
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4541589/
https://ncbi.nlm.nih.gov/pubmed/25992469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52771
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