An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Mesenchymal stem cells (MSCs) - usually obtained from bone marrow - often require expansion culture. Our protocol uses clinical grade urokinase to degrade clots in the bone marrow and release MSCs for further use. This protocol provides a rapid and inexpensive alternative to bone marrow resampling....

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Schlaefli, Philipp, Bertolo, Alessandro, Malonzo, Cherry, Poetzel, Tobias, Baur, Martin, Steffen, Frank, Stoyanov, Jivko
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2015
Fag:
Developmental Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4541539/
https://ncbi.nlm.nih.gov/pubmed/25938767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52694
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