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An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
Mesenchymal stem cells (MSCs) - usually obtained from bone marrow - often require expansion culture. Our protocol uses clinical grade urokinase to degrade clots in the bone marrow and release MSCs for further use. This protocol provides a rapid and inexpensive alternative to bone marrow resampling....
Gorde:
| Argitaratua izan da: | J Vis Exp |
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| Egile Nagusiak: | , , , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
MyJove Corporation
2015
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4541539/ https://ncbi.nlm.nih.gov/pubmed/25938767 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52694 |
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