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Active promoters give rise to false positive ‘Phantom Peaks’ in ChIP-seq experiments

Chromatin immunoprecipitation (ChIP) is widely used to identify chromosomal binding sites. Chromatin proteins are cross-linked to their target sequences in living cells. The purified chromatin is sheared and the relevant protein is enriched by immunoprecipitation with specific antibodies. The co-pur...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Jain, Dhawal, Baldi, Sandro, Zabel, Angelika, Straub, Tobias, Becker, Peter B.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4538825/
https://ncbi.nlm.nih.gov/pubmed/26117547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv637
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