Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents

BACKGROUND: Distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. Among existing methods, propidium monoazide (PMA)-qPCR is a promising way to quantify viable cells. However, its efficiency depends on the composition of the effluent, particularly on tota...

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Vydáno v:BMC Microbiol
Hlavní autoři: Desneux, Jérémy, Chemaly, Marianne, Pourcher, Anne-Marie
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2015
Témata:
Methodology Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4537567/
https://ncbi.nlm.nih.gov/pubmed/26276157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-015-0505-6
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