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Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents
BACKGROUND: Distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. Among existing methods, propidium monoazide (PMA)-qPCR is a promising way to quantify viable cells. However, its efficiency depends on the composition of the effluent, particularly on tota...
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| Publié dans: | BMC Microbiol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4537567/ https://ncbi.nlm.nih.gov/pubmed/26276157 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-015-0505-6 |
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