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Determination of the effective charge of a protein in solution by capillary electrophoresis.

This paper describes two methods to estimate the effective charge of a protein in solution by capillary electrophoresis and demonstrates these methods by using representative proteins. In one method, a "charge ladder"--a series of derivatives of a protein differing by known increments of charge but...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Gao, J, Gomez, F A, Härter, R, Whitesides, G M
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1994
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45369/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7991578/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.25.12027
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