Determination of the effective charge of a protein in solution by capillary electrophoresis.
This paper describes two methods to estimate the effective charge of a protein in solution by capillary electrophoresis and demonstrates these methods by using representative proteins. In one method, a "charge ladder"--a series of derivatives of a protein differing by known increments of charge but...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45369/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7991578/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.25.12027 |
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