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Reducing amplification artifacts in high multiplex amplicon sequencing by using molecular barcodes

BACKGROUND: PCR amplicon sequencing has been widely used as a targeted approach for both DNA and RNA sequence analysis. High multiplex PCR has further enabled the enrichment of hundreds of amplicons in one simple reaction. At the same time, the performance of PCR amplicon sequencing can be negativel...

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Detaylı Bibliyografya
Yayımlandı:BMC Genomics
Asıl Yazarlar: Peng, Quan, Vijaya Satya, Ravi, Lewis, Marcus, Randad, Pranay, Wang, Yexun
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: BioMed Central 2015
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4528782/
https://ncbi.nlm.nih.gov/pubmed/26248467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-1806-8
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