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Reducing amplification artifacts in high multiplex amplicon sequencing by using molecular barcodes
BACKGROUND: PCR amplicon sequencing has been widely used as a targeted approach for both DNA and RNA sequence analysis. High multiplex PCR has further enabled the enrichment of hundreds of amplicons in one simple reaction. At the same time, the performance of PCR amplicon sequencing can be negativel...
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| Publicado no: | BMC Genomics |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4528782/ https://ncbi.nlm.nih.gov/pubmed/26248467 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-1806-8 |
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