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Primer ID Validates Template Sampling Depth and Greatly Reduces the Error Rate of Next-Generation Sequencing of HIV-1 Genomic RNA Populations

Validating the sampling depth and reducing sequencing errors are critical for studies of viral populations using next-generation sequencing (NGS). We previously described the use of Primer ID to tag each viral RNA template with a block of degenerate nucleotides in the cDNA primer. We now show that l...

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Pubblicato in:J Virol
Autori principali: Zhou, Shuntai, Jones, Corbin, Mieczkowski, Piotr, Swanstrom, Ronald
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4524263/
https://ncbi.nlm.nih.gov/pubmed/26041299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00522-15
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