Primer and platform effects on 16S rRNA tag sequencing

Sequencing of 16S rRNA gene tags is a popular method for profiling and comparing microbial communities. The protocols and methods used, however, vary considerably with regard to amplification primers, sequencing primers, sequencing technologies; as well as quality filtering and clustering. How resul...

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Bibliographic Details
Published in:Front Microbiol
Main Authors: Tremblay, Julien, Singh, Kanwar, Fern, Alison, Kirton, Edward S., He, Shaomei, Woyke, Tanja, Lee, Janey, Chen, Feng, Dangl, Jeffery L., Tringe, Susannah G.
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2015
Subjects:
Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4523815/
https://ncbi.nlm.nih.gov/pubmed/26300854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.00771
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