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Ultra-large field-of-view two-photon microscopy

We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to m...

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Detalhes bibliográficos
Publicado no:Opt Express
Main Authors: Tsai, Philbert S., Mateo, Celine, Field, Jeffrey J., Schaffer, Chris B., Anderson, Matthew E., Kleinfeld, David
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4523368/
https://ncbi.nlm.nih.gov/pubmed/26072755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.23.013833
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