Quantifying domain-ligand affinities and specificities by high-throughput holdup assay

Many protein interactions are mediated by small linear motifs interacting specifically with defined families of globular domains. Quantifying the specificity of a motif requires measuring and comparing its binding affinities to all its putative target domains. To this aim, we developed the high-thro...

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Bibliografiske detaljer
Udgivet i:Nat Methods
Main Authors: Vincentelli, Renaud, Luck, Katja, Poirson, Juline, Polanowska, Jolanta, Abdat, Julie, Blémont, Marilyne, Turchetto, Jeremy, Iv, François, Ricquier, Kevin, Straub, Marie-Laure, Forster, Anne, Cassonnet, Patricia, Borg, Jean-Paul, Jacob, Yves, Masson, Murielle, Nominé, Yves, Reboul, Jérôme, Wolff, Nicolas, Charbonnier, Sebastian, Travé, Gilles
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4521981/
https://ncbi.nlm.nih.gov/pubmed/26053890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3438
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