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Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters

Large-scale sequencing of prokaryotic (meta)genomic DNA suggests that most bacterial natural product gene clusters are not expressed under common laboratory culture conditions. Silent gene clusters represent a promising resource for natural product discovery and the development of a new generation o...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Montiel, Daniel, Kang, Hahk-Soo, Chang, Fang-Yuan, Charlop-Powers, Zachary, Brady, Sean F.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4517240/
https://ncbi.nlm.nih.gov/pubmed/26150486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1507606112
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