Isolation of the yeast histone octamer.
Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, pe...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45160/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7972003/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.23.11032 |
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