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An ultra-tolerant database search reveals that a myriad of modified peptides contributes to unassigned spectra in shotgun proteomics

Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even...

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Detaylı Bibliyografya
Yayımlandı:Nat Biotechnol
Asıl Yazarlar: Chick, Joel M., Kolippakkam, Deepak, Nusinow, David P., Zhai, Bo, Rad, Ramin, Huttlin, Edward L., Gygi, Steven P.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2015
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4515955/
https://ncbi.nlm.nih.gov/pubmed/26076430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3267
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