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A set of powerful negative selection systems for unmodified Enterobacteriaceae

Creation of defined genetic mutations is a powerful method for dissecting mechanisms of bacterial disease; however, many genetic tools are only developed for laboratory strains. We have designed a modular and general negative selection strategy based on inducible toxins that provides high selection...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Khetrapal, Varnica, Mehershahi, Kurosh, Rafee, Shazmina, Chen, Siyi, Lim, Chiew Ling, Chen, Swaine L.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4513841/
https://ncbi.nlm.nih.gov/pubmed/25800749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv248
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