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CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment
Trypanosoma cruzi is the etiologic agent of Chagas disease, and current methods for its genetic manipulation have been highly inefficient. We report here the use of the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system for disrupting genes in t...
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| Published in: | mBio |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society of Microbiology
2015
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4513075/ https://ncbi.nlm.nih.gov/pubmed/26199333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01012-15 |
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